Investigation of Serum Crosslinked N-telopeptides of Type I Collagen (NTx) Levels and Total Antioxidant Capacity in Patients with Type 2 Diabetes Mellitus

Purpose: Our aim was to investigate the serum cross-linked N-telopeptides of type I collagen levels and total antioxidant capacity in patients with type 2 diabetes mellitus. Methods: 46 patients with type 2 diabetes mellitus (24M, 22F) aged 41 to 68 years (54.36 ± 7.92) and 34 healthy controls (17M, 17F) aged 40 to 69 years (52.91 ± 8.04) were enrolled in the study. Serum cross-linked N-telopeptides of type I collagen levels were measured by enzyme-linked immunosorbent assay method using a commertially available kit, total antioxidant capacity levels were measured by a colorimetric method based on the oxidation of 2,2’-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) radical and hemoglobin A1c levels were measured by high performance liquid chromatography technique. Results and Conclusion: Serum cross-linked N-telopeptides of type I collagen levels of the patients were significantly higher (p<0.01) whereas total antioxidant capacity levels were significantly lower (p<0.05) than those of the controls. Also, there was a significant positive correlation between serum cross-linked N-telopeptides of type I collagen and hemoglobin A1c levels (r=0.301, p<0.05) and a significant negative correlation between total antioxidant capacity and hemoglobin A1c levels (r=-0.382, p<0.01) in the patients group. In conclusion, our results show patients with type 2 diabetes mellitus have a significantly reduced total antioxidant capacity levels and there is a risk of bone resorption in these patients which can be estimated by measuring serum cross-linked N-telopeptides of type I collagen levels